Aquatic Animal Health (Fish Pathology/ Aquatic Microbiology)

M. F. Sc. DISSERTATION

Title: Efficacy of Bamboo [Melocanna baccifera (Roxburgh) Kurz, 1875] Extract in Labeo Rohita (Hamilton, 1822) Fingerlings against Fungal Infection under Low pH Stress

Objectives:

1. to perform in vitro evaluation of the properties of bamboo (Melocanna baccifera) extract;
2. to evaluate the toxicity of most efficient extract in Labeo rohita fingerlings;
3. to evaluate the pathophysiological responses of L. rohita fingerlings fed with bamboo (M. baccifera) extract; and
4. to evaluate the response of bamboo (M. baccifera) extract fed L. rohita fingerlings challenged with fungal infection under low pH stress

The work involves preparation of extracts (aqueous and alcoholic), phytochemical profiling, antioxidant and antimicrobial properties through in vitro tests. Toxicity study was carried through feed on L. rohita fingerlings using BLAL extract. Haemato-immuno-biochemical parameters of L. rohita fingerlings were also observed for 60 days when administered through BLAL extract based feed. Regular sampling was done at 7^th, 15^th, 30^th and 60^th day of feeding. Different haematological parameters like RBC, WBC, PCV, Hb, MCV, MCH, MCHC; biochemical parameters like blood glucose, ALP, GPT, GOT, plasma Na⁺, K⁺, Fe²⁺ Ca⁺, Mg⁺ and immunological parameters like lysozyme activity, NBT, anti-protease activity, myeloperoxidase activity plasma protein, immunoglobulin, albumin, albumin: globulin and immunegene (HSP70, IL-1𝛽, TNF-𝛼) expression were studied. Challenge study was also carried where fish were exposed to multiple stressors like low pH stress, Saprolegnia parastica infection, S. parastica infection under low pH and Aeromonas hydrophila infection after feeding with BLAL extract based feed for 7 days.

1. Participated in “Hands on Structural Bioinformatics and Data Mining Techniques” 24-26 september 2017 sponsored by Dept. of Biotechnology, Govt. of India and organized by COF-CAU, Tripura, India.
2. Participated in “National Conference on Linking Startup and SMEs in Food Processing with Government Schemes and Markets” 28 November 2017 sponsored by ASSOCHAM, India and organised by COF-CAU, Tripura, India.
3. Participated in 29^th All India Congress of Zoology, International Symposium on Culture Based Fisheries in Inland Open water and Satellite Symposium on Fish Immunology, 9-11 June 2017. Organised by ICAR-CIFRI, Barrackpore, West Bengal, India.
4. Participated and presented poster in International Symposium on Aquatic Animal Health and Epidemiology for Sustainable Asian Aquaculture, 20-21 Aril, 2017. Organised by ICAR-NBFGR, Lucknow, Uttar Pradesh, India.
5. Participated and presented paper in 2^nd Student Project Programe on Changes of Climate Change, 17-18 March 2017. Organised by Holy Cross College, Lembucherra, Tripura, India.
6. Participated in hands-on-trainings on “Soil and Water Quality Management in Aquaculture” organised by COF-CAU, Tripura, India.
7. Participated in National Seminar on ‘Fisheries and Aquaculture: Livelihood Security, Sustainability and Conservation’, 21-22 January 2016. Organised by NESFA and AFSIB in collaboration with COF-CAU, Tripura, India.
8. Participated in hands-on-trainings on “Capacity Building in Molecular Biology and Bioinformatic Techniques” sponsored by Dept. of Biotechnology, Govt. of India and organised by COF-CAU, Tripura, India.
9. Participated in “Hands on Bioinformatics and Molecular Biology Techniques applied to Fisheries and Aquaculture” sponsored by Dept. of Biotechnology, Govt. of India and organised by COF-CAU, Tripura, India.
10. Participated in “Hand on Training on Fish Disease Diagnosis and Management” sponsored by ICAR-NBFGR, Lucknow and NFDB, Hyderabad and organised by COF-CAU, Tripura, India.
11. Participated in “In Plant Training in Fisheries Post-Harvest Technology” from 17 Jan to 7 Feb 2015 at NIFPTT, Cochin, Kerala, India.
12. Participated in training of “Fish Harvest Technology” from 9-13 Feb 2015 at CIFNET, Cochin, Kerala, India.
13. Hand on training of “Fish Seed Production and Rearing” from 25 Feb to 31 March 2015 at Baghel Fisheries, Durg, India.

1. Microbiological techniques
2. Fish pathogens, their diagnosis and control
3. Determination of haemato-immuno-biochemical parameters of blood
4. Determination of antimicrobial, antioxidant and phytochemical profiling of plant extract
5. PCR and molecular expression analysis
6. Fish breeding, hatchery and farm management
7. Adept at software like SPSS, MS office, BIOST and handling and analysis of experimental data.

1. Participated in CAU 4^th inter-collegiate games and sports meet 2017 at COF-CAU, Tripura, India (Badminton).
2. Participated in State level inter-collegiate and university science and drama competition, 2016 at Iswarchandra Vidyasagar College, Belonia, Tripura, India.
3. Participated in ICAR 14^th all India inter agricultural university youth festival 2013-14 at UAS, Bangalore, India (Skit, Mime and Elocution).
4. Participated and winner in various college level competition Football, elocution, debate, extempore, shot-put.
5. Participated in Chhattisgarh state level competition for schools, 2004-05 at Durg, CG, India (Cricket)
6. Participated in National Service Scheme (NSS) camp at Bhagatpur, Kawardha, CG

• Member of “North Eastern Society for Fisheries and Aquaculture (NESFA)” for North East States of India - COF, CAU, Tripura, India.

RESEARCH PAPER/ ARTICLE PUBLBISHED

LEAFLET

1. Bamboo leaf Extract in Aquafeed- A New Initiative (I. R. Khan, R. K. Saha and H. Saha)

ABSTRACT PUBLISHED

1. Khan, I. R., Saha, R. K., Saha, H. Prospect of Muli bamboo [Melocanna baccifera (Roxb.)] as a Phytotherapeutic Aquaculture. In International Symposium on Aquatic Animal Health and Epidemiology for Sustainable Asian Aquaculture, 2017.
2. Khan, I. R., Saha, R. K., Saha, H., Ijardar, R. S., Yadav, K. K. and Chauriya, B. Review on NF-kB - A Cell Signaling Pathway. In 29^th All India Congress of Zoology, International Symposium on Culture Based Fisheries in Inland Open water and Satellite Symposium on Fish Immunology, 2017.
3. Khan, I. R., Saha, R. K., Saha, H., Ijardar, R. S., Yadav, K. K. and Chauriya, B. RNA Interference (RNAi): A Therapeutic Strategy for Aquaculture. In 29^th All India Congress of Zoology, International Symposium on Culture Based Fisheries in Inland Open water and Satellite Symposium on Fish Immunology, 2017.
4. Singh, M., Khan, I. R., Saha, R. K. New Drug to Treat Fungal Disease Outbreak due to the Impact of Climate Change in Tropical Fish, Labeo rohita (Hamilton). In 2^nd student project programe on changes of climate change, 2017.
5. Yadav, K. K., Dhanze, R. and Khan, I. R., Chauriya, B., Ijardar, R. S. Prospect and Potential of Canal Based Culture Fisheries in India. In 29^th All India Congress of Zoology, International Symposium on Culture Based Fisheries in Inland Open water and Satellite Symposium on Fish Immunology, 2017.
6. Chandravanshi, S. K., Dhar, B., Majumdar, R. K. and Khan, I. R. Retort Pouch Processing for Development of Fish Sausage in Curry from Pangasius. In 29^th All India Congress of Zoology, International Symposium on Culture Based Fisheries in Inland Open water and Satellite Symposium on Fish Immunology, 2017.