ManuScript Details
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Paper Id:
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IJCIRAS1725
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Title:
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PREPARATION OF COLLAGEN PEPTIDE CROSS-LINKED 3D GELLAN GUM HYDROGEL SCAFFOLD FOR IN-VITRO ARTICULAR CHONDROCYTE CULTURE AND CARTILAGE TISSUE REGENERATION
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| Published in: |
International Journal Of Creative and Innovative Research In All Studies |
| Publisher: |
IJCIRAS |
| ISSN: |
2581-5334 |
| Volume / Issue: |
Volume 3 Issue 9 |
| Pages: |
9
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| Published On: |
6/13/2021 9:51:16 PM (MM/dd/yyyy) |
| PDF Url: |
http://www.ijciras.com/PublishedPaper/IJCIRAS1725.pdf |
Main Author Details
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Name:
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Anju Rana Magar |
| Institute: |
College of Biomedical Engineering and Applied sciences |
Co - Author Details
| Author Name |
Author Institute |
| Sandipa Khadka |
College of Biomedical Engineering and Applied sciences |
| Soniya Shahu |
College of Biomedical Engineering and Applied sciences |
| Sabita Laya Magar |
College of Biomedical Engineering and Applied sciences |
| Nisha Shrestha |
College of Biomedical Engineering and Applied sciences |
| Saurav Sharma |
College of Biomedical Engineering and Applied sciences |
| Mishal Pokhare |
College of Biomedical Engineering and Applied sciences |
| Rabina Lamichhane |
College of Biomedical Engineering and Applied sciences |
Abstract
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Research Area:
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Bio-engineering/ Technology |
| KeyWord: |
3D, scaffold, gellan gum, polysaccharide, MTT assay, collagen peptide, chondrocytes, culture |
| Abstract: |
Human Articular Cartilage is an avascular structure, which, when injured, has very slow healing capacity as compared to other tissues. Therefore, innovative ideas like tissue engineering have been a promising approach to facilitate regeneration of hyaline-like articular cartilage to avoid continued pain, arthrodesis, or joint arthroplasty. Obtaining appropriate cells for implantation; directing the development of those cells on a chondrogenic pathway using growth factors and/or cytokines; supporting the growing cells on a three-dimensional matrix; and having that matrix remain in the defected cartilage, at least until the cartilage is healed are the key factors for successful tissue engineering. In this respect, nature-derived hydrogel scaffolds have huge potential for cartilage tissue engineering, mimicking the native cellular microenvironment. This project demonstrates that the three-dimensional (3D) scaffold made up of a polysaccharide called gellan gum and collagen peptide can be used for in vitro culture of chondrocytes. The hydrogel of two different compositions were prepared out of which one was gellan gum hydrogel and the other one was collagen peptide crosslinked gellan gum hydrogel to be used as a scaffold. To compare the mechanical properties, swelling tests were performed and FTIR analysis was done to show the composition and crosslinking in the hydrogel. Then, human articular chondrocytes were isolated and encapsulated in each hydrogel and were cultured. Lastly, MTT assay was done to analyze cell viability and proliferation in each hydrogel which showed there was good proliferation of chondrocytes within the hydrogels. |
Citations
Copy and paste a formatted citation or use one of the links to import into a bibliography manager and reference.
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IEEE
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Anju Rana Magar, Sandipa Khadka, Soniya Shahu, Sabita Laya Magar, Nisha Shrestha, Saurav Sharma, Mishal Pokhare, Rabina Lamichhane, "PREPARATION OF COLLAGEN PEPTIDE CROSS-LINKED 3D GELLAN GUM HYDROGEL SCAFFOLD FOR IN-VITRO ARTICULAR CHONDROCYTE CULTURE AND CARTILAGE TISSUE REGENERATION", International Journal Of Creative and Innovative Research In All Studies,
vol. 3, no. 9, pp. 17-25, 2021.
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MLA
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Anju Rana Magar, Sandipa Khadka, Soniya Shahu, Sabita Laya Magar, Nisha Shrestha, Saurav Sharma, Mishal Pokhare, Rabina Lamichhane "PREPARATION OF COLLAGEN PEPTIDE CROSS-LINKED 3D GELLAN GUM HYDROGEL SCAFFOLD FOR IN-VITRO ARTICULAR CHONDROCYTE CULTURE AND CARTILAGE TISSUE REGENERATION." International Journal Of Creative and Innovative Research In All Studies,
vol 3, no. 9, 2021, pp. 17-25.
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APA
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Anju Rana Magar, Sandipa Khadka, Soniya Shahu, Sabita Laya Magar, Nisha Shrestha, Saurav Sharma, Mishal Pokhare, Rabina Lamichhane (2021). PREPARATION OF COLLAGEN PEPTIDE CROSS-LINKED 3D GELLAN GUM HYDROGEL SCAFFOLD FOR IN-VITRO ARTICULAR CHONDROCYTE CULTURE AND CARTILAGE TISSUE REGENERATION. International Journal Of Creative and Innovative Research In All Studies,
3(9), 17-25.
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PREPARATION OF COLLAGEN PEPTIDE CROSS-LINKED 3D GELLAN GUM HYDROGEL SCAFFOLD FOR IN-VITRO ARTICULAR CHONDROCYTE CULTURE AND CARTILAGE TISSUE REGENERATION
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