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Paper Id: IJCIRAS1443
Title: IN VITRO REGENERATION OF UNDER EXPLOITED ETHNO-MEDICINAL PLANT LIMONIA ACIDISSIMA L.
Published in: International Journal Of Creative and Innovative Research In All Studies
Publisher: IJCIRAS
ISSN: 2581-5334
Volume / Issue: Volume 2 Issue 6
Pages: 8
Published On: 11/8/2019 8:45:11 PM      (MM/dd/yyyy)
Main Author Details
Name: chitralekha saini
Institute: MN College and Research Institute
Co - Author Details
Author Name Author Institute
Sapna Tyagi MN College and Research Institute
Abstract
Research Area: Plant culture
KeyWord: Cotyledons internodal segments, , epicotyls, hypocotyls, in vitro culture, Limonia acidissima L.
Abstract: In the present study, a protocol was optimized for establishment of callus and in vitro regeneration of medicinally important fruit tree Limonia acidissima L family Rutaceae for production of useful pharmaceuticals & conservation of the plant. Various explants viz. Epicotyl, hypocotyl, cotyledon and intrenodal segment were obtained by in vitro seed germination and established on MS medium supplemented with various concentrations and combinations of kinetin, BAP and 2,4-D. Best callusing response was observed on MS medium supplemented with 2,4-D (1.0mg/L) in combination with kinetin (0.5mg/L). Internodal segments showed best shooting response on MS medium supplemented with BAP (0.5mg/L) and Kinetin (0.5mg/L). In vitro rooting was done on different concentrations of IAA. 1.5 mg/l IAA was found to be best for rooting. The in vitro cultures developed could be exploited for production of pharmaceuticals and large scale production of this medicinally important tree.
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IEEE
chitralekha saini, Sapna Tyagi, "In Vitro regeneration of under exploited ethno-medicinal Plant Limonia acidissima L.", International Journal Of Creative and Innovative Research In All Studies, vol. 2, no. 6, pp. 1-8, 2019.
MLA chitralekha saini, Sapna Tyagi "In Vitro regeneration of under exploited ethno-medicinal Plant Limonia acidissima L.." International Journal Of Creative and Innovative Research In All Studies, vol 2, no. 6, 2019, pp. 1-8.
APA chitralekha saini, Sapna Tyagi (2019). In Vitro regeneration of under exploited ethno-medicinal Plant Limonia acidissima L.. International Journal Of Creative and Innovative Research In All Studies, 2(6), 1-8.
In Vitro regeneration of under exploited ethno-medicinal Plant Limonia acidissima L.
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