ManuScript Details
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Paper Id:
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IJCIRAS1120
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Title:
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ANTIBIOTIC SUSCEPTIBILITY STUDY ON GRAM POSITIVE BACTERIA BIOFILMS
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| Published in: |
International Journal Of Creative and Innovative Research In All Studies |
| Publisher: |
IJCIRAS |
| ISSN: |
2581-5334 |
| Volume / Issue: |
Volume 1 Issue 9 |
| Pages: |
12
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| Published On: |
2/26/2019 2:52:27 AM (MM/dd/yyyy) |
| PDF Url: |
http://www.ijciras.com/PublishedPaper/IJCIRAS1120.pdf |
Main Author Details
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Name:
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Saurav Sharma |
| Institute: |
College of Biomedical Engineering and applied Sciences, Hadigaun, Kathmandu 44600, Nepal |
Co - Author Details
| Author Name |
Author Institute |
| Mishal Pokharel |
College of Biomedical Engineering and applied Sciences, Hadigaun, Kathmandu 44600, Nepal |
Abstract
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Research Area:
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Microbiology |
| KeyWord: |
Biofilm, Antibiotics, Staphylococcus, Enterococcus
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| Abstract: |
Bacteria in a biofilm have greater capability in overcoming antibiotic attack with biofilm bacteria being 10 to 1,000-fold more antibiotic resistant as compared to their planktonic counterparts. As a consequence, infections involving biofilms present particular problems to manage effectively. Current studies of biofilms in the presence of antibiotics have been performed under full aerobic conditions and after 24-48 hours of biofilm formation. Neither of these two parameters reflects the in-vivo state of the biofilms. Biofilm formation inside the body require longer periods and might be weeks before the clinical symptoms indicate the presence of infection, furthermore there are few places in the human body where a biofilm is exposed to the levels of oxygen present in atmosphere. These local environment differences are likely to influence antibiotic activity. Earlier studies have confirmed that planktonic cells show different responses to antibiotics under different environments. This research aims to study the process of biofilm formation by Staphylococcus and Enterococcus bacteria and their response to antibiotics (Vancomycin, Teicoplanin, Linezolid, Dalbavancin, Telavancin, Daptomycin and Tigecycline) using different concentrations under different environmental conditions, thus reflecting the in-vivo conditions. Microtiter plate based system was used to grow biofilms; biofilm biomass and activity of biofilm bacteria were estimated using the Crystal violet and MTT assay respectively. Minimum inhibitory concentrations (MIC) of planktonic cells were analyzed experimentally. Biofilms were tested with different concentrations of antibiotics that were calculated with reference to their planktonic counterpart at each environment. Experimental results revealed significant effect of antibiotics to the planktonic cells but no significant variations were observed for their biofilms. However some significant variances were found in the antibiotic activity at different experimental environment; further improvements in the methodology must be done in order to validate these results. |
Citations
Copy and paste a formatted citation or use one of the links to import into a bibliography manager and reference.
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IEEE
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Saurav Sharma, Mishal Pokharel, "ANTIBIOTIC SUSCEPTIBILITY STUDY ON GRAM POSITIVE BACTERIA BIOFILMS", International Journal Of Creative and Innovative Research In All Studies,
vol. 1, no. 9, pp. 92-103, 2019.
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MLA
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Saurav Sharma, Mishal Pokharel "ANTIBIOTIC SUSCEPTIBILITY STUDY ON GRAM POSITIVE BACTERIA BIOFILMS." International Journal Of Creative and Innovative Research In All Studies,
vol 1, no. 9, 2019, pp. 92-103.
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APA
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Saurav Sharma, Mishal Pokharel (2019). ANTIBIOTIC SUSCEPTIBILITY STUDY ON GRAM POSITIVE BACTERIA BIOFILMS. International Journal Of Creative and Innovative Research In All Studies,
1(9), 92-103.
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ANTIBIOTIC SUSCEPTIBILITY STUDY ON GRAM POSITIVE BACTERIA BIOFILMS
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